Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV
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چکیده
منابع مشابه
Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV.
In the present study, we describe the development of a DNA vaccine against chicken anemia virus. The VP1 and VP2 genes of CAV were amplified and cloned into pBudCE4.1 to construct two DNA vaccines, namely, pBudVP1 and pBudVP2-VP1. In vitro and in vivo studies showed that co-expression of VP1 with VP2 are required to induce significant levels of antibody against CAV. Subsequently, the vaccines w...
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Chicken anemia virus was detected by PCR in tissue samples collected from poultry flocks in Gujarat,India. The VP1, VP2 and VP3 gene sequences of CAV from Anand, Gujarat were obtained after cloning thePCR products in pDrive cloning vector. Nucleotide sequence alignment with other CAV sequencesdemonstrated overall identity of 95-98.8%, 98.8-99.8% and 98.8-100% for VP1, VP2 and VP3 regions,respec...
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متن کاملcloning and sequence analysis of vp1, vp2 and vp3 genes of indian chicken anemia virus
chicken anemia virus was detected by pcr in tissue samples collected from poultry flocks in gujarat,india. the vp1, vp2 and vp3 gene sequences of cav from anand, gujarat were obtained after cloning thepcr products in pdrive cloning vector. nucleotide sequence alignment with other cav sequencesdemonstrated overall identity of 95-98.8%, 98.8-99.8% and 98.8-100% for vp1, vp2 and vp3 regions,respec...
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ژورنال
عنوان ژورنال: Comparative Immunology, Microbiology and Infectious Diseases
سال: 2011
ISSN: 0147-9571
DOI: 10.1016/j.cimid.2010.11.006